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modified wright-giemsa diff quick  (Baxter Dade Diagnostic)
90
Baxter Dade Diagnostic modified wright-giemsa diff quick
Modified Wright Giemsa Diff Quick, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diff-quick  (Baxter Dade Diagnostic)
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Baxter Dade Diagnostic diff-quick
Diff Quick, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diff-quick method dade diagnostics aquada  (Baxter Dade Diagnostic)
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Baxter Dade Diagnostic diff-quick method dade diagnostics aquada
Diff Quick Method Dade Diagnostics Aquada, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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modified wright-giemsa stain  (Baxter Dade Diagnostic)
90
Baxter Dade Diagnostic modified wright-giemsa stain
(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed <t>with</t> <t>methanol,</t> and stained with a modified <t>Wright-Giemsa</t> stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
Modified Wright Giemsa Stain, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/modified wright-giemsa stain/product/Baxter Dade Diagnostic
Average 90 stars, based on 1 article reviews
modified wright-giemsa stain - by Bioz Stars, 2026-04
90/100 stars
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diff-quick kit  (Baxter Dade Diagnostic)
90
Baxter Dade Diagnostic diff-quick kit
(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed <t>with</t> <t>methanol,</t> and stained with a modified <t>Wright-Giemsa</t> stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
Diff Quick Kit, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diff-quick kit/product/Baxter Dade Diagnostic
Average 90 stars, based on 1 article reviews
diff-quick kit - by Bioz Stars, 2026-04
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diff-quick aquada  (Baxter Dade Diagnostic)
90
Baxter Dade Diagnostic diff-quick aquada
(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed <t>with</t> <t>methanol,</t> and stained with a modified <t>Wright-Giemsa</t> stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
Diff Quick Aquada, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
diff-quick aquada - by Bioz Stars, 2026-04
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cytocentrifuge  (Thermo Fisher)
90
Thermo Fisher cytocentrifuge
(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed <t>with</t> <t>methanol,</t> and stained with a modified <t>Wright-Giemsa</t> stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
Cytocentrifuge, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cytocentrifuge - by Bioz Stars, 2026-04
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diff-quick stain  (Baxter Dade Diagnostic)
90
Baxter Dade Diagnostic diff-quick stain
(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed <t>with</t> <t>methanol,</t> and stained with a modified <t>Wright-Giemsa</t> stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).
Diff Quick Stain, supplied by Baxter Dade Diagnostic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diff-quick stain/product/Baxter Dade Diagnostic
Average 90 stars, based on 1 article reviews
diff-quick stain - by Bioz Stars, 2026-04
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Image Search Results


(a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

Journal:

Article Title: Enhanced Susceptibility to Pulmonary Infection with Burkholderia cepacia in Cftr −/− Mice

doi: 10.1128/IAI.69.8.5138-5150.2001

Figure Lengend Snippet: (a and b) Total and differential cell counts in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia (either BC7 or ATCC 25416). BAL fluid was harvested from each mouse by washing with three 1-ml aliquots of PBS via a cannulated trachea. Cells from 100 μl of BAL fluid were cytocentrifuged onto a slide, fixed with methanol, and stained with a modified Wright-Giemsa stain. Total numbers of cells and different cell types were determined by counting a minimum of 300 cells/slide. (a) Total numbers of cells; (b) differential cell counts. Bars, cell averages from two (ATCC 25416) and seven (PBS and BC7) animals in each group. SEM were calculated only for groups that had more than four animals. Asterisk, statistically significant difference between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (c) Surface expression of myeloid marker Ly-6G (Gr-1) on cells recovered in BAL fluid from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe). (d and e) Assessment of neutrophil activation in cells recovered by BAL. Shown is oxidant production, as indicated by surface expression of β2 integrin CD11b (d) and oxidation of dihydrorhodamine (e) and by neutrophils recovered by BAL from Cftr+/+ and Cftr−/− mice treated with PBS or B. cepacia isolate BC7. Analysis was done by flow cytometry as outlined in Materials and Methods. Asterisk, statistically significant differences between the groups as determined by ANOVA with correction for multiple comparisons (Sheffe).

Article Snippet: Fifty microliters of BAL fluid was sedimented in a cytocentrifuge (Shandon Inc., Pittsburgh, Pa.), fixed with methanol, and stained using a modified Wright-Giemsa stain (Diff-Quick; Dade Diagnostics, Aquanda, Puerto Rico).

Techniques: Staining, Modification, Giemsa Stain, Expressing, Marker, Flow Cytometry, Activation Assay